Brenda Raissa

Graduated in Pharmacy from Instituto Educacional Santo Agostinho (2015). She
participated in research projects related to quality control of medicines and
medicinal plants. He completed an extra-curricular internship at Faculdade Santo
Agostinho (856 hours), being responsible for organizing the laboratories, preparing
solutions and reagents and assisting in research for the institution's students' final
coursework. She worked in a private pharmacy in the Municipality of Berizal-MG as a
pharmacist responsible for ensuring the management of inputs related to the
Pharmacy, through the development of procedures and team guidance, as well as
providing primary care to patients, aiming at the availability of resources, standards
quality and effectiveness of treatment. He is currently a Master in the Multicenter
Postgraduate Program in Biochemistry and Molecular Biology at Faculdade Federal
de São João Del-Rei where he worked with molecular aspects of tumor evasion of
the immune system mediated by detrital cells promoted by the breast cancer cell
line MDA-MB- 231. She is also a PhD student in the Postgraduate Program in
Biochemistry and Immunology at the Federal University of Minas Gerais, with PhD
defense prediction and obtaining the title in June 2024 online. She has additional
training in data science through courses available on the coursera platform in
Python and R and is interested in expanding her knowledge of the broad area of
data science applied to oncology. Currently, she is a student in a MBA on Data
Science e Analytics at the University of São Paulo (USP).

-Ensure the management of inputs related to the Pharmacy, through the
development of procedures and team guidance
- Provide primary care to patients, aiming at the availability of resources, quality
standards and treatment effectiveness.

Thesis decription

• MDA-MB-231 cell line culture to evaluate the potential antitumor activity of a
peptide isolated from scorpion venom.

Purification of the synthetic peptide using the HPLC device, and using these
peptide to biological assays.
• Determination IC50 by resazurin method, fluorescence microscopy preparation,
and the electronic microscopy previous preparation for fluorescence
microscopy and electronic microscopy analyses.
• Images processing analisys using softawares cell pose, cell profiler and Fiji/
Image J
• The results indicate that the peptide acts via the plasma membrane in the MDAMB-
231 cell line.
• The deadline to complete the PhD is around july/2024, but at the moment I am
only writing the thesis and articles related the thesis.

Dissertation description:

Dissertation description:
During the master degree I elucidated a new mechanism related to dendritic cells,
differentiated from healthy donor monocytes (mo-DCs), immune response tumor
invasion through exosomes.
The MDA-MB-231 breast cancer cell line released exosomes able to change mature
dendritic cell phenotype, modulating the it’s action to stimulate the T lymphocyte
proliferation.
The phenotype profile of dendritic cells was performed by flow cytometry at UFMG.
In addition, exosomes of MDA-MB-231 cell line express MicroRNAs relate to mo-DC
response modulation, and mo-DCs after MDA-MB-231 cell line exosomes presented
apoptosis genes expression. The miR and apoptosis genes expression were
evaluated by RT-qPCR.